Invitrogen™ Qubit™ microRNA Assay Kit, 500 Assay

Invitrogen™ Qubit™ microRNA Assay Kit, 500 Assay

The Qubit microRNA Assay Kit allows easy and accurate quantification of microRNA (miRNA) using the Qubit Fluorometer, even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for miRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng in the assay tube following the supplied protocol below, even in the presence of mRNA.

The Qubit microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer. Because the assay tolerates 1–20 µL of sample in an assay volume of 200 µL, accurate results can be obtained for initial sample concentrations from 50 ng/mL–100 µg/mL.

Features

• Accurately detects as little as 0.5 ng miRNA in the assay tube
• Core dynamic range of 5–500 ng/mL
• Accurate results for sample concentrations from 50 ng/mL to 100 µg/mL
• For Use With (Equipment): Qubit™ Fluorometer
• Sample Type: RNA (<40 bases), Tissue (FFPE), Total RNA, miRNA, mRNA (Bacterial), Total RNA (Yeast), Total RNA (Bacterial), mRNA

Contents

• Qubit® microRNA Reagent (Component A): 1.25 mL 250 μL
• Qubit® microRNA Buffer (Component B): 250 mL 50 mL
• Qubit® microRNA Standard #1 (Component C): 5 mL 1 mL
• Qubit® microRNA Standard #2 (Component D): 0 × 500 μL 4 × 250 μL