Invitrogen™ RiboMinus™ Bacteria 2.0 Transcriptome Isolation Kit

Invitrogen™ RiboMinus™ Bacteria 2.0 Transcriptome Isolation Kit

Highly efficient depletion of 16S, 23S, and 5S rRNA from a variety of species
The RiboMinus Bacteria 2.0 Transcriptome Isolation Kit is a complete and automatable kit that enables efficient and selective removal of bacterial and archaebacterial ribosomal RNA (rRNA) from intact total RNA (100 ng to 5 μg) in less than two hours.

Features

• Efficient—the included RiboMinus Pan-Prokaryote Probe Mix has consistently shown less than 10% of reads mapping to 16S and 23S rRNA reads
• Wide range of targets—designed with >150 probe designs targeting 16S, 23S, and 5S rRNA targets from >76 representative species of bacteria and archaebacteria from common
• phyla and sub-phyla
• Convenient—Dynabeads MyOne Streptavidin C1 magnetic beads and bead cleanup module and protocols included for Kingfisher instruments
• Flexible— accommodates mass inputs from 100 ng to 5 µg of total RNA; works with wide range of total RNA samples with RIN values from 5.0–10.0
• High detection sensitivity—increases the number of sequencing reads mapping to protein-coding transcripts compared to previous version
• Suitable for metatranscriptomics—deplete rRNA of many microbial species in metagenomic samples

Contents

• RiboMinus™ Pan-Prokaryote Probe Mix:  40 μL
• Hybridization Buffer:  2 × 1.6 mL
• RiboMinus™ Magnetic Beads:  2 X 3 mL
• Binding Solution Concentrate:  6 mL
• Nucleic Acid Binding Beads:  150 μL
• Wash Solution Concentrate:  2 mL