Invitrogen™ Cells-to-cDNA™ II Kit, 100 Reactions

Invitrogen™ Cells-to-cDNA™ II Kit, 100 Reactions
 

The Ambion® Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr. No RNA isolation is required. This kit contains sufficient reagents for 100 reactions and is ideal for those who want to perform reverse transcription reactions on small numbers of cells, numerous cell samples, or for scientists who are unfamiliar with RNA isolation.

Features

• No RNA purification required
• From cells in culture to cDNA in less than 2 hr
• Detect rare messages in as few as 1 cell
• Ideal for labs not equipped for RNA isolation

Contents

• PBS (pH 7.4): 1X40 mL
• Cell Lysis II Buffer: 10 mL
• DNase 1 (2 U/μL): 200 μL
• RT Buffer: 10X500 μL
• M-MLV Reverse Transcriptase: 100 μL
• RNase Inhibitor (10 U/μL): 100 μL
• dNTP Mix (2.5 mM each dNTP): 400 μL
• Random Decamers (50 μM): 200 μL
• Oligo(dT)18 Primers (50 μM): 200 μL
• Armored RNA® Contro: 120 μL
• Armored RNA® Primer Pair (10 μM e): 200 μL
• Endogenous Primer Pair (5 μM ea): 200 μL
• Nuclease-free Water: 3.5 mL