Invitrogen™ RNAqueous™-96 Total RNA Isolation Kit

Invitrogen™ RNAqueous™-96 Total RNA Isolation Kit

The RNAqueous®-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains sufficient reagents for 192 (2 x 96) RNA purifications. The RNAqueous®-96 Kit is ideal for the isolation of total RNA from multiple samples and very small samples.
The procedure uses an RNA-binding glass-fiber filter and either vacuum filtration or centrifugation methods to provide high yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. The 96-well plates are compatible with most common 96-well plate vacuum manifolds and 96-well plate centrifuge rotors. The RNAqueous®-96 Kit provides consistently high yields of RNA from various amounts of starting material. The high quality of RNA isolated is illustrated.
The RNAqueous®-96 Automated Kit (AM1812) is the same product in a format suitable for automation.

Features

• Format: 96-well plate
• High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
• Final Product Type: Total RNA
• For Use With (Equipment): 96-Well Plate Centrifuge Rotors, Vacuum Manifold (96-Well Plate)
• Sample Type: Mammalian Cells, Tissue (Mammalian)

Contents

• Nuclease-free Water: 50 mL
• Elution Solution: 50 mL
• Filter Plates: 2
• Collection Plates: 2
• Adhesive Plate Sealers: 2
• Plate Lid room temp: 2
• Lysis/Binding Solution: 50 mL
• Wash Solution Concentrate: 175 mL
• Rebinding Mix: 53 mL
• DNase I Buffer: 12 mL
• DNase I (20 units/μL): 1.25 mL