Invitrogen™ RNaseAlert™ QC System

Invitrogen™ RNaseAlert™ QC System

Ambion® RNaseAlert® QC System is a patent-pending technology detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. Contain sufficient reagents for 5 × 96 high throughput assays.
The RNaseAlert® QC System uses a novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength. Fluorescence can be readily detected with a filter-based or monochromator-based fluorometer. Since the fluorescence of the RNaseAlert® Substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RNaseAlert® Substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase®

Features

• Detects as little as 3.5 x 10-7 units (∼0.5 pg) of RNase A with a fluorometer
• Simple, straightforward 30 min assay
• Monitor RNase activity in column fractions during protein purification
• Use with DNaseAlert™ QC System to quantitatively detect both RNases and DNases in the same sample
  
  

Contents

• tubes RNaseAlert Substrate: 5
• 10X RNase Alert Buffer: 5 mL
• RNase A: 500 μL
• TE Buffer (pH 7.0): 6 mL
• RNaseZap® Solution: 250 mL
• Nuclease-free Water: 50 mL