Invitrogen™ RNaseAlert™ QC System v2

Invitrogen™ RNaseAlert™ QC System v2

The RNaseAlert® QC System v2 detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. This version contains a substrate that is ∼60% more sensitive than the previous version. The kit contains sufficient reagents for 5 x 96 (480) high-throughput assays.
The RNaseAlert® QC System v2 uses a new, more sensitive, novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength.


Features

• Detects as little as ∼0.3 pg of RNase A with a fluorometer
• Simple, straight-forward, 30-minute assay
• Designed to monitor RNase activity in column fractions during protein purification
• Use with DNaseAlert™ QC System to quantitatively detect both RNases and DNases in the same sample
  
  

Contents

• RNaseAlert® Substrate v2: 5 tubes
• 10X RNaseAlert® Buffer: 5 mL
• RNase A: 500 μL
• TE Buffer (pH 7.0): 6 mL
• RNaseZap® Solution: 250 mL
• Nuclease-free Water: 50 mL