Invitrogen™ RNaseAlert™ Lab Test Kit v2

Invitrogen™ RNaseAlert™ Lab Test Kit v2

RNaseAlert® Lab Test Kit v2 detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. This kit is suitable for testing small numbers of sample and ensuring that solutions, tubes, tips, etc. in your lab are RNase-free. This new version contains a more sensitive substrate that is ∼60% more sensitive than the previous version. The kit contains sufficient reagents for 25 reactions.
The RNaseAlert® Lab Test Kit v2 uses a new, more sensitive, novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength.

Features

• Detects as little as ∼0.3 pg of RNase A with a fluorometer
• Simple, straight-forward 30-minute assay
  

Contents

• RNaseAlert® Substrate v2: 25 tubes
• 10X RNaseAlert® Lab Test Buffer: 250 μL
• RNase A: 500 μL
• RNaseZap® Solution: 50 mL
• Nuclease-free Water: 1.75 mL