Invitrogen™RNAqueous™ Total RNA Isolation Kit

Invitrogen™RNAqueous™ Total RNA Isolation Kit

The RNAqueous® Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
How it works
The RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once

Features

• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples

Contents

• Formaldehyde Load Dye:   6 mL
• Lysis/Binding Solution:  60 mL
• Water for 64% Ethanol:  60 mL
• Lithium Chloride Precipitation Solution: • 4 mL
• Wash Solution 1:  40 mL
• Wash Solution 2/3 Concentrate:   80 mL
• Filter Cartridges:  50
• Collection Tubes:  100
• Elution Solution:  10 mL