Ion Torrent™ Ion AmpliSeq™ Mouse TCR Beta SR Assay, RNA

Ion Torrent™ Ion AmpliSeq™ Mouse TCR Beta SR Assay, RNA

The Ion AmpliSeq Mouse TCR Beta SR Assay is a robust, targeted next-generation sequencing (NGS) assay designed to accurately identify and measure the clonal expansion of T lymphocytes by targeting the complementarity-determining region 3 (CDR3) of the T-cell receptor (TCR) gene locus from total RNA input. The assay can be used for basic and translational research to identify T-cell clones since the nucleotide sequence of the CDR3 region is unique to each T cell and codes for the part of the TCR beta chain that is involved in antigen recognition.

Features

• Compatibility with a vast array of research sample types, including FFPE tissue, fresh-frozen (FF) tissue, whole blood, PBLs, and PBMCs
• High sensitivity and low limit of detection (LoD) for rare clone identification through dual-barcode indexing
• Efficient workflow with 48 hour sample-to-results time
• Flexible input requirements ranging from 25 ng to 1 µg
• Streamlined and user-friendly informatics solution with with multi-sample analysis functionality

Contents

• Ion AmpliSeq TCRB-SR Assay, RNA
• Ion AmpliSeq Library Kit Plus
• Ion NGS RT Kit