Ion Torrent™ Ion Total RNA-Seq Kit v2, 12 Reactions

Ion Torrent™ Ion Total RNA-Seq Kit v2, 12 Reactions

The Ion Total RNA-Seq Kit v2 includes the reagents needed to prepare representative cDNA libraries for strand-specific RNA sequencing on the Ion GeneStudio™ S5, Ion Proton™, or the Ion Personal Genome Machine™ (PGM™) systems. The Ion GeneStudio S5 and Ion Proton systems are ideally suited for sequencing the whole transcriptome (ribosomal RNA depleted or polyA), while the Ion GeneStudio S5 and Ion PGM systems are suitable for viral and bacterial transcriptomes. Version 2 of the Ion Total RNA-Seq Kit is an improvement over the first generation kit. Magnetic bead-based purification replaces all of the filter cleanup steps and the total reaction time has been reduced to 6 hours.

Features

• Greater accuracy—SuperScript® VILO™ and Platinum® PCR SuperMix High Fidelity added for highest template fidelity
• Barcode compatible—works with Ion Xpress™ RNA-Seq Barcode 01-16 Kit for multiplexing
• Automation friendly—magnetic bead-based purification simplifies automation of library construction
• Preserves strand information—all mapped reads are aligned in the direction of transcription relative to the chromosomal strand
• For Use With (Equipment): Ion PGM™ System, Ion Proton™ SystemAllows you to analyze any type of RNA—supports small RNA, rRNA depleted total RNA, and poly(A) RNA
• Libraries: cDNA Library
• Sequencing Type: Transcriptome Sequencing
• Workflow Step: NGS Library Generation
• Sample Type: RNA

Contents

• 10X RT Buffer 48 reactions: 1 tube,
•  2.5 mM dNTP Mix 48 reactions: 1 tube
• Platinum HiFi MasterMix 48 reactions: 1 tube
• 10x Superscript Enzyme Mix 48 reactions: 1 tube
• Ion 3'-PCR-primer v2 48 reactions: 1 tube
• Ion 5'-PCR-primer v2 48 reactions: 1 tube
• Ion 5'-PCR-primer v2 48 reactions: 1 tube
• Ion RT Primer v2 48 reactions: 1 tube
• Ion Adaptor Mix v2 48 reactions: 1 tube
• Binding Soluntion Concentrated 48 reactions: 1 bottle
• Wash Soln Concentrated 48 reactions: 1 bottle
• Nucleic Acid Binding Beads 48 reactions: 1 bottle