Ion Torrent™ Oncomine™ Breast cfDNA Research Assay v2

Ion Torrent™ Oncomine™ Breast cfDNA Research Assay v2

The Oncomine Breast cfDNA Assay v2 is part of a complete solution to detect breast tumor-derived DNA (ctDNA) in cell-free DNA (cfDNA). It provides the reagents for library construction and a single pool of multiplex PCR primers for preparation of amplicon libraries from cfDNA obtained from the plasma fraction of a single 10 mL tube of whole blood.

The Oncomine Breast cfDNA Assay v2 enables the analysis of: Hotspot genes: AKT1, EGFR, ERBB2, ERBB3, ESR1, FBXW7, KRAS, PIK3CA, SF3B1, TP53 (∼152 hotspots) - Copy number genes (CNVs): CCND1, ERBB2, FGFR1 - Full length genes: TP53 (∼80% coverage)

Features

• Optimized amplicon design for short cfDNA ensures highest possible capture rate
• Optimized targeted assay design allows highly multiplexed next-generation sequencing (NGS), reducing sequencing costs per sample
• Efficient workflow in just two days

Contents

• 16 µL breast cfTNA panel
• 320 µL cfDNA library PCR master mix
• 832 µL low TE buffer
• 8 µL cfDNA library primer P1
• 8 µL cfDNA library primer A/BC1