Ion Torrent™ Oncomine Lung Cell-Free Total Nucleic Acid Research Assay

Ion Torrent™ Oncomine Lung Cell-Free Total Nucleic Acid Research Assay

The Oncomine Lung cfTNA Research Assay is part of a complete solution to detect lung tumor-derived cell-free DNA and RNA (cell-free total nucleic acid; cfTNA) isolated from the plasma fraction of whole blood. It provides the reagents for library construction and a single pool of multiplex PCR primers for preparation of amplicon libraries from cfTNA obtained from the plasma fraction of a single 10 mL tube of whole blood.

The Oncomine Lung cfTNA Research Assay enables the analysis of: Hotspot genes (SNVs) and short indels: ALK, BRAF, EGFR, ERBB2, KRAS, MAP2K1, MET, NRAS, PIK3CA, ROS1, and TP53 (∼168 hotspots covered) - Gene fusions: ALK, RET, ROS1 - MET exon 14 skipping - Copy number gene (CNV): MET

Features

• Optimized amplicon design for short cfDNA ensures highest possible capture rate
• Tag Sequencing technology minimizes false positives by removing randomly incorporated errors
• Optimized targeted assay design allows highly multiplexed next-generation sequencing (NGS), reducing sequencing costs per sample
• Efficient 2-day workflow

Contents

• 16 µL lung cfTNA panel
• 320 µL cfDNA library PCR master mix
• 832 µL low TE buffer
• 8 µL cfDNA library primer P1
• 8 µL cfDNA library primer A/BC1
• 22 µL SuperScript VILO Master Mix