Ion Torrent™ Oncomine™ IGH FR3(d)-J Assay, DNA

Ion Torrent™ Oncomine™ IGH FR3(d)-J Assay, DNA

The Oncomine IGH FR3(d)-J Assay is a targeted next-generation sequencing (NGS) assay for detection of B-cell diversity to measure clonal expansion/contraction with accuracy and for detection of rare clones with high sensitivity. Primers have been specifically designed to target the distal region of FR3 to J of the immunoglobulin heavy chain of the B-cell receptor. Due to lower levels of somatic hypermutation observed in the distal region of FR3, there is an increase in primer-binding efficiency. When combined with Ion Reporter Software, data can be easily analyzed and reports run within a short period of time.

For use with equipment: GeneStudio.

Features

• Sequencing of FR3(d)-J region of IGH chain
• Unique primer design to distal FR3 region may reduce primer mismatches due to somatic hypermutation
• Detect rare clones at LOD of 10-6
• Optimized Oncomine informatics for easy, all-inclusive analysis and publication-ready data plots

Contents

• Oncomine IGH FR3(d)-J Panel
• dNTP mix
• Ion AmpliSeq Library Plus Kit