mirVana™ miRNA Isolation Kit, with Phenol

mirVana™ miRNA Isolation Kit, with Phenol

The mirVana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method. The method isolates total RNA ranging in size from kilobases down to 10-mers. Each kit contains sufficient reagents and consumables for either 40 isolations of total RNA (including small RNAs), or 20 separate large and small RNA fractions.

Features

• Efficient isolation of small RNA-containing total RNA
• Enrich for small RNA (<200 nt) to increase sensitivity in downstream analyses
• Simple 30 minute procedure
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis
• Compatible with virtually all cell and tissue types

Contents

• miRNA Wash Solution I: 30 mL (room temperature)
• Wash Solution 2/3: 50 mL (room temperature)
• Collection Tubes: 80 (room temperature)
• Filter Cartridges: 40 (room temperature)
• Lysis/Binding Buffer: 100 mL (4°C)
• miRNA Homogenate Additive: 10 mL (4°C)
• Acid-Phenol Chloroform: 100 mL (4°C)
• Gel Loading Buffer II: 1.4 mL (-20°C)
• Elution Solution: 5 mL (-20°C, 4°C, or room temperature)