Presto™ Midiprep Plasmid DNA Kit, 25 Preps

Presto™ Midiprep Plasmid DNA Kit, 25 Preps

The Presto™ Midi Plasmid Kit was designed for Plasmid DNA Purification from 50-150 ml of cultured bacterial cells using an efficient plasmid midiprep system. For processing smaller volumes, the Presto™ Mini Plasmid Kit is also available. Both plasmid kits include TrueBlue Lysis Buffer (an optional color indicator) to prevent common handling errors, while ensuring efficient cell lysis and neutralization. The Presto™ Midi Plasmid Kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound by the plasmid midi column and the contaminants are removed with PMC Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 80 minutes without ultracentrifuges, HPLC or other toxic reagents.

Contents

• PM1, PM2, PM3 Buffers
• TrueBlue Lysis Buffer
• PEQ Buffer
• PMC Buffer
• PEL Buffer
• RNase A
• Filter Columns (yellow spin columns)
• Plasmid Midi Columns (anion exchange white resin columns)