Thermo Scientific™ 1-Step Human High-Yield Maxi IVT Kit, 10 x 100 µL
On demandThermo Scientific™ 1-Step Human High-Yield Maxi IVT Kit, 2 x 100 µL
The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.
The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression in an overnight incubation of up to 750 µg/mL. The complete maxi-scale kit includes all the components required for transcription and translation of a recombinant gene, including an optimized expression vector.
Features
- High expression—up to 750 µg/mL of expressed protein
- Reproducibility—low variability between experiments
- Fast—express high levels of protein with 6 hours to overnight incubation
- Functional—obtain functionally active proteins, including those containing disulfide bonds
Contents
Sufficient For: 10 reactions of 100 µL each
- HeLa Lysate: 500 µL
- Accessory Proteins: 100 µL
- Reaction Mix: 200 µL
- 5X Dialysis Buffer: 3 mL
- Positive Control DNA (pCFE-GFP): 10 µg
- pT7CFE1-NHis-GST-CHA: 10 µg
- Maxi dialysis Device: 10 each
- Nuclease-free Water: 3 x 5 mL
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Thermo Scientific™ 1-Step Human High-Yield Maxi IVT Kit, 2 x 100 µL
$ On demand