Thermo Scientific™ 1-Step Human High-Yield Maxi IVT Kit, 10 x 100 µL

Thermo Scientific™ 1-Step Human High-Yield Maxi IVT Kit, 2 x 100 µL

The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.

The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression in an overnight incubation of up to 750 µg/mL. The complete maxi-scale kit includes all the components required for transcription and translation of a recombinant gene, including an optimized expression vector.

Features

• High expression—up to 750 µg/mL of expressed protein
• Reproducibility—low variability between experiments
• Fast—express high levels of protein with 6 hours to overnight incubation
• Functional—obtain functionally active proteins, including those containing disulfide bonds

Contents

Sufficient For: 10 reactions of 100 µL each

• HeLa Lysate: 500 µL
• Accessory Proteins: 100 µL
• Reaction Mix: 200 µL
• 5X Dialysis Buffer: 3 mL
• Positive Control DNA (pCFE-GFP): 10 µg
• pT7CFE1-NHis-GST-CHA: 10 µg
• Maxi dialysis Device: 10 each
• Nuclease-free Water: 3 x 5 mL