Invitrogen™ EasySelect™ Pichia Expression Kit

Invitrogen™ EasySelect™ Pichia Expression Kit

The EasySelect™ Pichia Expression Kit provides all needed components for protein production in the yeast Pichia pastoris. The EasySelect™ Kit contains Pichia yeast strains, expression vectors, and reagents that enable transformation of Pichia cells. Selection with Zeocin™ antibiotic makes it easy to screen for high-copy number transformed variants.

Features

• Culture Pichia cells as easily as E. coli
• Obtain higher cell density than other microbial expression systems.
• Generate more protein per cell than other microbial expression systems.
• Benefit from eukaryotic protein processing, protein folding, and post-translational modifications.
• Easily scale-up from shake flasks up to industrial-scale fermentation.

Contents

The EasySelect Pichia Expression Kit includes:

• Plasmids pPICZ A, B, & C and pPICZα A, B, & C: 20 µg each 
• Pichia pastoris strains X-33 (wild-type), GS115 (his4), KM71H (arg4 aox1::ARG4)
• Control strains for both intracellular and secreted expression
• Zeocin™ antibiotic; 250 mg
• Media (YP and YNB)
• EasyComp™ Pichia Transformation Kit
• 5´ AOX1, 3´ AOX1: 2 µg each
• α-factor sequencing primers