Thermo Scientific™ Active Rho Pull-Down and Detection Kit

Thermo Scientific™ Active Rho Pull-Down and Detection Kit

The Thermo Scientific Pierce Active Rho Pull-Down and Detection Kit enables selective enrichment and detection of GTP-bound Rho GTPase through specific protein interaction with the Rhotekin protein-binding domain.

The Active Rho Pull-Down and Detection Kit includes purified GST-Rhotekin Rho-binding domain (RBD), glutathione agarose resin, positive and negative controls (GTPγS and GDP, respectively), lysis/binding/washing buffer, anti-Rho antibody, secondary antibody, sample buffer, spin columns and collection tubes. The kit was validated using lysates from NIH 3T3 cells, a cell line known to have robust Rho activity.

Features

• Highly sensitive and accurate—optimized reagents, specific pan anti-Rho antibody and Western blot procedure ensure accurate controls and semi-quantitative results
• Validated—functionally tested for Rho detection to ensure quality and performance
• Compatible—effective with a variety of cell types from mouse, rat and human sources

Contents

• GST-Rhotekin-RBD: 3 vials
• Anti-Rho Antibody, 5 units: 75 µL 
• Stabilized Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated: 2 mL 
• Glutathione Agarose Resin: 3 mL 
• GDP (100X): 50 µL 
• GTPyS (100X): 50 µL 
• SDS Sample Buffer (2X): 1.5 mL 
• Lysis/Binding/Wash Buffer (1X): 100 mL 
• Spin Cups: 30 cups 
• Collection Tubes: 90 tubes