Thermo Scientific™ Pierce™ HA-Tag Magnetic IP/Co-IP Kit

Thermo Scientific™ Pierce™ HA-Tag Magnetic IP/Co-IP Kit

The Thermo Scientific Pierce Magnetic HA-Tag IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of HA fusion proteins or co-IP experiments using HA-tagged bait proteins.

For use with equipment: KingFisher™ Flex, Magnetic Stand

Features

• Specific magnetic beads—covalently immobilized high-quality anti-HA monoclonal antibody enables high yields of immunoprecipitation products
• Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding
• Trouble-free elution—low-pH elution buffer ensures recovery of HA-tagged protein interaction complexes without antibody leaching contamination
• Convenient and fast—complete kit and easy-to-follow instructions provide optimized protocols to perform IP or Co-IP experiment in approximately 1 hour
• Versatile—magnetic beads are compatible with manual and automated magnetic separation workflows (e.g., Thermo Scientific KingFisher Instruments)

Contents

• HA-tagged Positive Control (Part No. 26180X): 500 µL
• Pierce Anti-HA Magnetic Beads: 1 mL
• Pierce IP Lysis/Wash Buffer: 2 × 50 mL
• Non-Reducing Sample Buffer (5X): 5 mL
• Elution Buffer (pH 2.0): 5 mL
• Neutralization Buffer (pH 8.5): 1 mL