Thermo Scientific™ Pierce™ HA-Tag Magnetic IP/Co-IP Kit
On demand
Order number :
88838
Thermo Scientific™ Pierce™ HA-Tag Magnetic IP/Co-IP Kit
The Thermo Scientific Pierce Magnetic HA-Tag IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of HA fusion proteins or co-IP experiments using HA-tagged bait proteins.
For use with equipment: KingFisher™ Flex, Magnetic Stand
Features
- Specific magnetic beads—covalently immobilized high-quality anti-HA monoclonal antibody enables high yields of immunoprecipitation products
- Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding
- Trouble-free elution—low-pH elution buffer ensures recovery of HA-tagged protein interaction complexes without antibody leaching contamination
- Convenient and fast—complete kit and easy-to-follow instructions provide optimized protocols to perform IP or Co-IP experiment in approximately 1 hour
- Versatile—magnetic beads are compatible with manual and automated magnetic separation workflows (e.g., Thermo Scientific KingFisher Instruments)
Contents
- HA-tagged Positive Control (Part No. 26180X): 500 µL
- Pierce Anti-HA Magnetic Beads: 1 mL
- Pierce IP Lysis/Wash Buffer: 2 × 50 mL
- Non-Reducing Sample Buffer (5X): 5 mL
- Elution Buffer (pH 2.0): 5 mL
- Neutralization Buffer (pH 8.5): 1 mL
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