Thermo Scientific™ AminoLink™ Reductant
On demandThermo Scientific™ AminoLink™ Reductant
Thermo Scientific AminoLink Reductant (sodium cyanoborohydride) promotes the formation of stable bonds between aldehyde- and amine-containing molecules, enabling efficient labeling, conjugation, and immobilization of proteins and other molecules. Carbonyl groups such as aldehydes, ketones, and glyoxals react spontaneously with amino groups to form Schiff base intermediates that are in equilibrium with their free forms. The interaction is pH-dependent, being somewhat more efficient in acidic conditions, and especially strong at high pH. Addition of AminoLink Reductant to a reaction in which Schiff base formation has occurred results in complete reduction of the labile Schiff base intermediate to a chemically stable bond. Unlike sodium borohydride, sodium cyanoborohydride is sufficiently mild to avoid adversely reducing aldehydes to nonreactive hydroxyls.
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