Thermo Scientific™ AminoLink™ Immobilization Kit

Thermo Scientific™ AminoLink™ Immobilization Kit

The AminoLink Immobilization Kit contains AminoLink Coupling Resin and associated reagents sufficient to perform 5 protein immobilizations, with expected an expected yield of 2 to 20 mg protein per column. AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.

Features

• AminoLink Coupling Resin—aldehyde-activated crosslinked, 4% beaded agarose
• Ideal for antibodies and other proteins—immobilize molecules via primary amines (-NH2)
• Flexible coupling conditions—efficient (>85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent)
• Stable, permanent immobilization—Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
• Better than immobilization to CNBr-activated agarose—bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
• Versatile and reusable—prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

Contents

• AminoLink Columns, 2mL: 5 columns
• AminoLink Coupling Buffer (pH 7.0): 250 mL
• Quenching Buffer: 50 mL
• Wash Buffer: 240 mL
• Sodium Cyanoborohydride Solution, 5M: 0.5 mL
• Column Accessories