Thermo Scientific™ B-PER™ with Enzymes Bacterial Protein Extraction Kit, 250 mL

Thermo Scientific™ B-PER™ with Enzymes Bacterial Protein Extraction Kit, 250 mL

Thermo Scientific B-PER with Enzymes Bacterial Protein Extraction Kit is an easy-to-use cell lysis reagent is a nonionic detergent-based solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation. All B-PER Reagents are compatible with downstream applications, such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay). Depending on the particular application, protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.

Features

• Tris buffered formulation with lysozyme and DNase enzymes
• Improved cell membrane solubilization and DNA digestion
• Ready to use—one-step cell lysis of gram-positive and gram-negative bacteria using a mild, nonionic detergent (proprietary) in Tris or phosphate buffer formulations
• Fast and simple—just add B-PER Reagent to a bacterial pellet, incubate with mixing for 10 to 15 minutes and recover soluble proteins after pelleting the cell debris
• Convenient – B-PER Complete Reagent contains lysozyme and a universal nuclease in a single formulation with 4°C storage
• Excellent yields—recover recombinant proteins from bacterial lysates or purify inclusion bodies to near-homogeneous levels
• Flexible—B-PER Reagents are suitable for any scale of protein extraction and are available in phosphate and 1X and 2X Tris formulations, with and without enzymes
• Compatible—completely compatible with addition of protease inhibitors, and the resulting protein extract can be used in protein assays, typical affinity purification methods (e.g., GST, 6xHis) and other applications

Contents

Sufficient For: 125g cell paste

• B-PER Reagent, 250 mL
• Lysozyme, 50 mg/mL, 0.5 mL
• DNase I, 2500 U/mL, 0.5 mL