Thermo Scientific™ HisPur™ Cobalt Purification Kit, 3 mL
On demand
Order number :
90092
Thermo Scientific™ HisPur™ Cobalt Purification Kit, 3 mL
The Thermo Scientific HisPur Cobalt Purification Kit contains 0.2-ml HisPur spin columns, buffers, and collecting tubes for the purification of polyhistidine-tagged proteins. The HisPur Cobalt Resin in these columns is a tetradentate chelating agarose resin charged with divalent cobalt (Co2+) for obtaining high-purity his-tagged proteins with no metal contamination.
Features
- High purity—obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
- Specificity—cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
- Low metal leaching—no metal contamination in eluted histidine-tagged protein sample
- Versatility—purify proteins under native or denaturing conditions; compatible with Thermo Scientific Pierce Cell Lysis Reagents and a variety of buffer additives
- Cost effective—resin can be reused several times or discarded after single use
- Flexibility—available as bulk resin, kits, predispensed columns, chromatography cartridges and 96-well filter plates
- Format: 50 mL conical, Kit, Spin Column
- Stationary Phase: Metal Chelate, Divalent Cobalt
Contents
Sufficient For: Purifying ∼30 mg of His-tagged protein per column
- HisPur Cobalt Spin Columns, 3 mL, 5 columns
- Equilibration/Wash Buffer, 225 mL
- Elution Buffer, 75 mL
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