Thermo Scientific™ Blocker™ BSA (10X) in PBS

Thermo Scientific™ Blocker™ BSA (10X) in PBS

Blocker BSA (10X) is a purified 10% solution of bovine serum albumin for blocking in western blotting, ELISA, IHC, and nucleic acid detection methods.

Blocker BSA is a 10% (w/v) solution of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. It is most frequently diluted 10-fold (to 1% BSA) in 1X PBS or 1X TBS for initial testing. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid of endogenous biotin.

Contents

• 10% (w/v) bovine serum albumin in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4