Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate), 1 g
On demand
Order number :
21586
Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate), 1 g
Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.
BS3 contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.
Features
- Reactive groups: sulfo-NHS ester (both ends)
- Reactive towards: amino groups (primary amines)
- Amine-reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
- Water-soluble; compare with DSS
- Membrane-impermeable, allowing for cell surface labeling
- High-purity, crystalline reagent can be used to create high-purity crosslinked conjugates
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