Thermo Scientific™ EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride), 10 x 1 mg
On demandThermo Scientific™ EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride), 10 x 1 mg
Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.
Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.
Features
- Reactive group: carbodiimide
- Reaction target: activates carboxyl groups to conjugate to amino groups (primary amines)
- Several conjugation strategies—react EDC alone with target groups or include NHS or Sulfo-NHS to increase reaction efficiency or to stabilize active intermediate for later reaction to amines
- Neutral linkage—forms neutral amide bonds between carboxyls and amines
- Water-soluble reagent—add directly to reactions in aqueous, physiological buffers
- Soluble reaction byproducts—easily removed by washing with water or dilute acid
- High-purity, crystalline reagent—use to create high-quality, activated derivatives
There are no specifications
There are no report
You May Also Like
![](https://laboshop.ae/public/storage/files/2dc51e86-fa8e-4f94-86ac-c6570b78b1cd.jpg)
Invitrogen™ Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/100a6297-ff18-4f96-8346-e9f96280c52c.jpg)
Thermo Scientific™ MBS (m-maleimidobenzoyl-N-hydroxysuccinimide ester)
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/e7841d59-f862-40db-a919-48ff297e4deb.jpg)
Thermo Scientific™ TMTsixplex™ Isobaric Mass Tagging Kit, 35 Reactions
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/0f951c0b-aeb2-4307-b2b3-d714a33ce7f0.jpg)
Invitrogen™ Click-iT™ sDIBO Alkynes for copper-free click chemistry, Alexa Fluor™ 488
$ On demand