Thermo Scientific™ BS3-d4 (bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4)
On demand
Order number :
21595
Thermo Scientific™ BS3-d4 (bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4)
Thermo Scientific Pierce BS3-d0/d4 reagents form a matched set of heavy (deuterated) and light mass varieties of BS3 amine-reactive crosslinker that facilitates the identification of crosslinked peptides by mass spectrometry.
The BS3-d0/d4 reagents are mass-analogs of the popular BS3 crosslinker (Part No. 21580). The d-zero variety (BS3-d0) possesses ordinary hydrogen atoms while the d-four variety (BS3-d4) contains four deuterium atoms that make it exactly four hydrogen mass units heavier. When the two varieties are mixed and used to crosslink interacting proteins, distinctive mass patterns result that enable unambiguous identification of the crosslinked molecules.
Features
- Deuterium-labeled BS3 and its standard hydrogen-only analog—specially manufactured for crosslinking protein structure:function analysis by mass spectrometry
- Easy identification of crosslinked fragments—peptide masses differing by 4 units unambiguously identify crosslink at specific loci within a protein interaction structure
- Application-specific instructions—help guide the first time user through the application
- Method requires only microgram amounts of protein—excellent alternative to NMR or X-ray crystallography-based methods that require large amounts of protein, special solvents or crystal formation
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