Thermo Scientific™ CaptureSelect™ AAV9 Ligand Leakage ELISA, 1 Assay

Thermo Scientific™ CaptureSelect™ AAV9 Ligand Leakage ELISA, 1 Assay

The CaptureSelect AAV9 Ligand Leakage ELISA detects affinity ligand contamination of adeno-associated viruses (AAV) purified using POROS CaptureSelect AAV9 Affinity Resin. The ligand, a 13 kD recombinant protein, is coupled by multipoint attachment through stable amide bonds to give high chemical stability and low leakage. Even when covalently attached, small amounts of the affinity ligand can leach off of the chromatography support and co-elute with the purified AAV. This sensitive ELISA detects affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. The CaptureSelect™ AAV9 Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

For use with equipment: Microplate Reader