Thermo Scientific™ CaptureSelect™ Albumin RoboColumn

Thermo Scientific™ CaptureSelect™ Albumin RoboColumn

CaptureSelect™ Human Albumin Affinity Matrix, available here in RoboColumn™ format, has been specifically designed for the purification of human albumin and albumin fusion proteins. RoboColumns are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

Features of this affinity matrix include:

• Highly selective for human albumin

• Unique elution characteristics allowing for gentle elution of albumin fusion proteins

• Excellent scalability

• Non-animal-derived

The high selectivity and yields obtained using CaptureSelect Human Albumin Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step. The mild elution conditions make the resin suitable for the purification of albumin fusion proteins, especially those that are pH sensitive.

Free of animal components

Our CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single domain antibody fragments. The ligand is a 13 kDa single domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Characteristics:

Matrix: agarose-based, aldehyde-activated

Average particle size: 65 ± 10 µm

Ligand: CaptureSelect HSA affinity ligand

Ligand coupling method: aldehyde coupling via NH2 residues of the ligand

Binding capacity: ∼15 mg albumin/mL resin

Elution conditions (neutral pH): 20 mM Tris, pH 7.4 with 2 M MgCl2 or with 1 M NaCl + 50%(v/v) propylene glycol or with 1 M NaCl + 0.5 M L-arginine

Elution conditions (acidic pH): 0.1 M glycine, pH 3.0

Formulation buffer: 20% (v/v) ethanol

RoboColumn is a registered trademark of ATOLL GmbH.

Features

• Product Line: CaptureSelect™
• Immunoassay Kit Format: Agarose Bead
• Contents & Storage: Store in refrigerator (2–8°C).