Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose, 5 mL
On demand
Order number :
A36798
Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose, 5 mL
Thermo Scientific Pierce Anti-DYKDDDDK Magnetic Agarose provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins from in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to a magnetite-embedded agarose core particle.
Features
- Specific—unique base beads and highly specific antibody minimizes off-target binding (low non-specific binding)
- High purity—optimized bind-wash-elute protocol enables high purity
- High yield—special antibody conjugation method enables high yield
- Rapid—entire purification protocol typically takes less than 40 mins
- Economical—purification protocol allows multiple reuses
- Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)
Contents
- 5 mL settled beads. 20 mL supplied as a 25% suspension in PBS containing 0.02% sodium azide.
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