Thermo Scientific™ CaptureSelect™ C-tagXL Affinity Matrix, 50 mL

Thermo Scientific™ CaptureSelect™ C-tagXL Affinity Matrix, 50 mL

CaptureSelect C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix. It purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmatic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity recoveries of pH-sensitive target proteins. C-tagXL Affinity Matrix recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.

Features

• Mild elution, making it suitable for pH-sensitive proteins
• Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies)
• Excellent scalability
• Non-animal-derived