Thermo Scientific™ GlycoLink™ IP Kit

Thermo Scientific™ GlycoLink™ IP Kit

The Thermo Scientific GlycoLink IP Kit provides components for effective immunoprecipitation (IP and co-IP) based on small-scale, covalent, affinity-resin immobilization of antibodies and other glycoproteins via oxidized sugar groups.

The GlycoLink Kit uses hydrazide-activated UltraLink Resin to immobilize and perform 25 IP or Co-IP assays with different antibodies or glycoprotein ligands. The method is ideal for polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions. Monoclonal antibodies that contain adequate carbohydrates are also effective. Antibodies immobilized by this hydrazide-to-carbohydrate method have unobstructed antigen-binding sites and optimal purification capability. The prepared glycoprotein or antibody columns can be regenerated and reused at least five times for immunoprecipitation, co-immunoprecipitation or pull-down affinity-purification without significant loss in binding capacity.

Features

• Efficient immobilization—couple 2 to 10 µg of oxidized antibody or other glycoprotein to 20 µL of resin in 2 hours or less
• Stable immobilization—resonance structure of the hydrazone bonds are sufficiently stable so the antibody remains coupled during elution
• Preserves binding function—immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target
• Versatile applications—immobilize any molecule that contains oxidizable sugars, including glycoproteins for use in purifying protein interaction binding partners

Contents

Sufficient For: 25 IP/Co-IP reactions, each using 20 µL antibody-coupled resin

• UltraLink Hydrazide Resin: 0.5 mL
• GlycoLink Coupling Buffer: 50 mL
• Aniline: 0.2 mL
• Sodium meta-periodate: 0.5 g
• Wash Buffer: 60 mL
• Zeba Spin Desalting Columns: 0.5 mL, 25 columns
• IP Lysis/Wash Buffer: 50 mL
• PBS Pack (makes 500 mL): 1 pack
• Elution Buffer, pH 2.8: 50 mL
• Lane Marker Sample Buffer, Non-reducing, (5X): 5 mL
• Pierce Spin Columns-Screw Caps: 25 columns
• Collection Tubes: 2 mL, 100 tubes