Thermo Scientific™ CaptureSelect™ Factor X Ligand Leakage ELISA, 1 Assay
On demandThermo Scientific™ CaptureSelect™ Factor X Ligand Leakage ELISA, 1 Assay
The CaptureSelect Factor X Ligand Leakage ELISA detects possible contamination of purified product with Factor X affinity ligand after use of CaptureSelect Factor X Affinity Matrix.
The Factor X affinity ligand is a 13-kD recombinant protein expressed in yeast and covalently coupled to a chromatography matrix to give high chemical stability and low leakage. During the chromatography process small amounts of the affinity ligand can leach off of the chromatography support and co-elute with the protein. This sensitive leakage ELISA detects affinity ligand contamination of less than 1 ng/mL.
The CaptureSelect Factor X Ligand Leakage ELISA is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. It can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.
For use with equipment: Microplate Reader
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