Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))
On demand
Order number :
21565
Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))
Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.
Features
- Reactive groups: NHS ester (both ends)
- Reactive towards: amino groups (primary amines)
- Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
- Membrane-permeable, allowing for intracellular crosslinking
- Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
- Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS
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