Thermo Scientific™ EZ-Link™ Amine-PEG11-Biotin

Thermo Scientific™ EZ-Link™ Amine-PEG11-Biotin

Thermo Scientific EZ-Link Amine-PEG11-Biotin is a biotinylation reagent containing a long 11-unit polyethylene glycol (PEG) spacer arm and a terminal primary amine group for conjugation and protein labeling with EDC or other crosslinkers.

Amine-PEG2-Biotin and Amine-PEG3-Biotin are the shorter two of three amine-modified biotin compounds that contain polyethylene glycol (PEG) spacer arms. The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the—NH2 group of the amino-biotin, forming an amide bond.

Features

• Biotinylation—label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins
• Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers
• Pegylated—the 11-unit, polyethylene glycol (PEG) group in spacer arm enhances water solubility of biotinylated molecules
• Long reach—total reagent length is more than 50 angstroms, greatly minimizing steric hindrance for the biotin binding-function