Thermo Scientific™ EZ-Link™ Biocytin

Thermo Scientific™ EZ-Link™ Biocytin

Thermo Scientific EZ-Link Biocytin is a simple variant of biotin that contains a primary amine in its valeric acid chain, which provides the essential backbone for construction of certain long-chain and trifunctional biotinylation reagents.

Biocytin is ε-N-[d-biotinyl]-L-lysine, a compound formed by conjugation of the epsilon amine of lysine to the valeric acid side chain of biotin. It contains terminal carboxyl and amino groups, which provide functional handles for derivatization or conjugation to proteins, surfaces and other molecules. Carbodiimide (EDC) and NHS-ester crosslinker chemistries are most often utilized for covalent modifications involving biocytin. The compound is also useful as an amino acid control or biotin standard in assay methods involving streptavidin binding.

Features

• Biotinylation—label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins
• Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers
• Lysine derivative—functions as a biotin-tagged amino acid; potentially useful as a control or quenching reagent in biotin assay methods
• Medium length—spacer arm (total length added to target) is 20.1 angstroms, representing a 7-atom extension of the native biotin valeric acid