Thermo Scientific™ EZ-Link™ HPDP-Biotin, 10 x 1 mg

Thermo Scientific™ EZ-Link™ HPDP-Biotin, 10 x 1 mg

Thermo Scientific EZ-Link HPDP-Biotin is a membrane-permeable biotin labeling reagent that reacts with sulfhydryl (-SH) groups. The resulting disulfide bond between the target sulfhydryl molecule and the biotin group can be cleaved by reducing agents to release the biotin group and regenerate the protein (or peptide) in its original, unmodified form. Labeling with HPDP-Biotin is convenient when using immobilized avidin, streptavidin, or Thermo Scientific NeutrAvidin Protein to purify the target molecules for reducing SDS PAGE or mass analysis; the captured biotinylated molecules can be efficiently eluted from the support by cleaving the disulfide bond with dithiothreitol (DTT) or other reducing agent rather than by attempting to dissociate the high affinity interaction between avidin and biotin with strong acid or denaturant.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

Features

• Protein labeling—biotinylate antibodies or other proteins for use in protein methods
• Thiol-reactive—reacts with sulfhydryls (-SH), such as the side-chain of cysteine (C)
• Pyridyldithiol-activated—perform reactions at pH 6.5 to 7.5 in buffers such as PBS
• Reversible—forms disulfide bonds, which can be cleaved using DTT or other reducing agent
• Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Medium length—spacer arm (total length added to target) is 29.2 angstroms