Thermo Scientific™ EZ-Link™ Iodoacetyl-LC-Biotin

Thermo Scientific™ EZ-Link™ Iodoacetyl-LC-Biotin

Thermo Scientific EZ-Link Iodoacetyl-LC-Biotin is a mid-length, haloacetyl-activated, sulfhydryl-reactive biotinylation reagent that forms stable, irreversible thioether bonds at alkaline pH.

Iodoacetyl-LC-Biotin is a haloacetyl-biotin compound for labeling protein cysteines and other molecules that contain sulfhydryl groups. This reagent specifically reacts with reduced thiols (-SH) in alkaline buffers to form permanent (irreversible) thioether bonds. The unique feature of Iodoacetyl-LC-Biotin is its extended yet chemically simple hexylenediamine spacer arm.

Features

• Protein labeling—biotinylate antibodies or other proteins for use in protein methods
• Membrane-permeable—can be used to label inside cells (intracellular)
• Thiol-reactive—reacts with sulfhydryls (-SH), such as the side-chain of cysteine (C)
• Iodoacetyl-activated—perform reactions in the dark at pH 7.5 to 8.5 in Tris or borate buffer
• Irreversible—forms permanent thioether bonds; spacer arm cannot be cleaved
• Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Medium length—spacer arm (total length added to target) is 27.1 angstroms; contains hexylenediamine extension