Thermo Scientific™ EZ-Link™ Micro Sulfo-NHS-SS-Biotinylation Kit

Thermo Scientific™ EZ-Link™ Micro Sulfo-NHS-SS-Biotinylation Kit

The Thermo Scientific EZ-Link Sulfo NHS-SS Biotinylation Kit contains sufficient reagents for 8 biotinylation reactions (e.g., 0.05–0.2 mg antibody per reaction). The EZ-Link Sulfo-NHS-SS-Biotin included in the kit is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Features

• Protein labeling – biotinylate antibodies to facilitate immobilization, purification or detection
• Cell surface labeling – biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
• Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule
• Soluble – charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Medium length—spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain

Contents

Sufficient For: 8 labeling reactions, each with 0.05–0.2 mg of antibody

• EZ-Link Sulfo-NHS-SS-Biotin: 8 x 1 mg
• PBS Pack (makes 500 mL): 1 pack
• Zeba Spin Desalting Columns (7K MWCO): 2 mL, 10 columns