Thermo Scientific™ EZ-Link™ Micro Sulfo-NHS-LC-Biotinylation Kit

Thermo Scientific™ EZ-Link™ Micro Sulfo-NHS-LC-Biotinylation Kit

The Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotinylation Kit contains reagents sufficient for 8 biotinylation reactions (e.g., 0.05–0.2 mg antibody per reaction). The EZ-Link Sulfo-NHS-LC-Biotin reagent included in the kit is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins, and other molecules that have primary amines.

Features

• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Medium length—spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass

Contents

Sufficient For: 8 labeling reactions, each with 0.05–0.2 mg of antibody

• EZ-Link Sulfo-NHS-LC-Biotin: 8 x 1 mg
• PBS Pack (makes 500 mL): 1 pack
• Zeba Spin Desalting Columns (7K MWCO): 2 mL, 10 columns