Thermo Scientific™ EZ-Link™ NHS-Desthiobiotin

Thermo Scientific™ EZ-Link™ NHS-Desthiobiotin

Thermo Scientific EZ-Link NHS-Desthiobiotin is a short, membrane permeable, amine-reactive labeling reagent whose biotin-like group is elutable from streptavidin, making it ideal for intracellular protein labeling and purification.

NHS-Desthiobiotin is a variant of biotin that is activated as an NHS ester to covalently label primary amines (-NH2) of proteins or other molecules with desthiobiotin groups. The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative to NHS-Biotin (Part No. 20217) for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. This simplest and smallest variety of amine-reactive desthiobiotin reagent is uncharged and cell membrane permeable; therefore, NHS-Desthiobiotin can be used with intact cells for intracellular protein labeling.

Features

• Desthiobiotin – analog of biotin allows easy elution from streptavidin, an ideal feature for affinity purification applications
• Protein labeling – tag antibodies or proteins in purified or mixed samples to enable high recovery in pull-down assays with streptavidin beads
• Membrane-permeable – achieve intracellular labeling with intact cells because the small, uncharged reagent easily diffuses across cell membranes
• Amine-reactive – reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides