Thermo Scientific™ EZ-Link™ NHS-Biotin
On demandThermo Scientific™ EZ-Link™ NHS-Biotin
Thermo Scientific EZ-Link NHS-Biotin is the simplest NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for general intracellular labeling.
NHS-Biotin is N-hydroxysuccinimidobiotin. It is the shortest of three similar EZ-Link NHS-Biotin Reagents that enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules in solution. Differing only in their spacer arm lengths, the three NHS-ester reagents offer researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Because they are uncharged and contain simple alkyl-chain spacer arms, these biotin compounds are membrane-permeable and useful for intracellular labeling.
Features
- Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
- Membrane-permeable—can be used to label inside cells (intracellular)
- Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
- Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
- Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers
- Very short—spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only
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