Thermo Scientific™ EZ-Link™ NHS-PEG12-Biotin, 25 mg

Thermo Scientific™ EZ-Link™ NHS-PEG12-Biotin, 25 mg

Thermo Scientific EZ-Link NHS-PEG12-Biotin enables simple and efficient biotin labeling of antibodies, proteins, and any other primary amine–containing macromolecule. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule. For example, antibodies labeled with NHS-PEG4-Biotin exhibit less aggregation when stored in solution as compared to antibodies labeled with reagents having only hydrocarbon spacers. Specific labeling of cell surface proteins is another useful application for these water-soluble and membrane-impermeable reagents.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

Features

• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
• Pegylated—spacer arm contains a hydrophilic, 12-unit, polyethylene glycol (PEG) group
• Enhances solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Very long reach—spacer arm (total length added to target) is 56 angstroms; this reduces steric hindrance when binding to avidin molecules