Thermo Scientific™ EZ-Link™ NHS-SS-Biotin

Thermo Scientific™ EZ-Link™ NHS-SS-Biotin

Thermo Scientific EZ-Link NHS-SS-Biotin enables simple and efficient biotinylation of antibodies, proteins and other primary amine-containing molecules, as well as intracellular labeling.

NHS-SS-Biotin is succinimidyl 2-(biotinamido)-ethyl-1,3' -dithiopropionate, a compound that enables simple and efficient biotinylation of antibodies, proteins and other primary amine-containing molecules. The extended spacer arm (24.3 angstroms) of this reagent reduces steric hindrance associated with binding to avidin or other biotin-binding proteins and incorporates a reducing agent-cleavable disulfide bond (-S-S-) within the spacer, providing further versatility to the reagent.

Features

• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Membrane-permeable—can be used to label inside cells (intracellular)
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
• Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule
• Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Medium length—spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain