Thermo Scientific™ EZ-Link™ NHS-SS-Biotin
On demand
Order number :
21441
Thermo Scientific™ EZ-Link™ NHS-SS-Biotin
Thermo Scientific EZ-Link NHS-SS-Biotin enables simple and efficient biotinylation of antibodies, proteins and other primary amine-containing molecules, as well as intracellular labeling.
NHS-SS-Biotin is succinimidyl 2-(biotinamido)-ethyl-1,3' -dithiopropionate, a compound that enables simple and efficient biotinylation of antibodies, proteins and other primary amine-containing molecules. The extended spacer arm (24.3 angstroms) of this reagent reduces steric hindrance associated with binding to avidin or other biotin-binding proteins and incorporates a reducing agent-cleavable disulfide bond (-S-S-) within the spacer, providing further versatility to the reagent.
Features
- Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
- Membrane-permeable—can be used to label inside cells (intracellular)
- Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
- Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule
- Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers
- Medium length—spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain
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