Thermo Scientific™ EZ-Link™ Phosphine-PEG4-Desthiobiotin, No-Weigh™ Format

Thermo Scientific™ EZ-Link™ Phosphine-PEG4-Desthiobiotin, No-Weigh™ Format

Thermo Scientific EZ-Link Phosphine-PEG4-Desthiobiotin is a pegylated reagent whose biotin-like group is elutable from streptavidin, activated for chemoselective ligation and purification of azide targets tagged by metabolic labeling.

Features

• Desthiobiotin—analog of biotin allows easy elution from streptavidin, an ideal feature for affinity purification applications
• Chemoselective—the phosphine reactive group is specific in biological samples for azide-tagged molecules, ensuring specific capture and purification of labeled targets
• Compatible—Staudinger reaction chemistry proceeds efficiently in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
• PEG spacer—polyethylene glycol spacer arm helps maintain solubility of labeled molecules and decreases steric hindrance for affinity-binding to avidin, streptavidin or NeutrAvidin Protein
• Soluble—easily dissolves in water-miscible solvents (e.g., DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples