Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin

Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin

Thermo Scientific EZ-Link Psoralen-PEG3-Biotin enables biotinylation of DNA or RNA via UV-light-activated intercalation of the psoralen group with thymine- and other pyrimidine-containing bases to form a covalent bonds.

Psoralen-PEG3-Biotin is a photoactivatable reagent for biotinylation of DNA or RNA. Upon activation with UV-Light (∼350nm), the psoralen group reacts with thymine- and other pyrimidine-containing bases to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

Features

• Biomolecular labeling—biotinylate DNA or RNA (approximately 1 biotin per 10 to 20 bases) without interfering with hybridization
• Photo-reactive—covalent attachment occurs in the presence of UV light (∼350nm) for 10 to 30 minutes
• Psoralen-activated—reacts through a cyclo-addition mechanism with the 5,6 double bond in thymine- and other pyrimidine-containing bases
• Pegylated—spacer arm contains a hydrophilic, 3-unit, polyethylene glycol (PEG) group
• Enhances solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Long reach —spacer arm (total length added to target) is 36.9 angstroms, minimizing steric hindrance for binding interactions with streptavidin