Thermo Scientific™ TMS(PEG)12 Branched Methyl-PEG-NHS-Ester Reagent

Thermo Scientific™ TMS(PEG)12 Branched Methyl-PEG-NHS-Ester Reagent

Thermo Scientific Pierce TMS(PEG)12 is a three-branched, polyethylene glycol compound (3 times 12 PEG units) activated as an NHS ester for covalent pegylation of primary amines (e.g., lysines) on proteins or assay surfaces.

TMS(PEG)12 is the abbreviation for a branched trimethyl (TM) and succinimide ester (S) derivative of polyethylene glycol (PEG) for efficient and specific modification of primary amines. Each methyl-terminated PEG (mPEG) branch contains 12 ethylene glycol units. The three branches are attached to a 4-unit PEG stem that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at the distal end. The NHS ester is spontaneously reactive with primary amines (—NH2), providing for efficient PEGylation of proteins, peptides and other amine-containing molecules or surfaces.

Features

• NHS-activated for efficient PEGylation of primary amines at pH 7-9; reaction of NHS-ester group results in formation of stable, irreversible amide bonds
• Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Easy-to-follow instructions increase the likelihood of a successful outcome