Thermo Scientific™ Pierce™ Co-Immunoprecipitation Kit

Thermo Scientific™ Pierce™ Co-Immunoprecipitation Kit

The Thermo Scientific Pierce Co-Immunoprecipitation Kit provides covalent antibody immobilization and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.

Co-IP is a common approach to study protein:protein interactions that uses an antibody to immunoprecipitate the antigen (bait protein) and co-immunoprecipitate any interacting proteins (prey proteins). Traditional co-IP methods that use Protein A or G result in co-elution of the antibody heavy and light chains that may co-migrate with relevant bands, masking important results. The Pierce Co-IP Kit resolves this issue by covalently coupling antibodies onto an amine-reactive resin. The kit includes optimized buffers for protein binding and recovery, reagents to perform control experiments and efficient spin columns and collection tubes, which shorten the protocol and minimize handling and mixing.

Features

• Works for any purified antibody—perform co-IP experiments using any purified antibody regardless of species or Ig class (chicken IgY, human IgE, mouse IgG1, IgM, etc.); no dependence on Protein A and Protein G affinity binding
• No antibody interference—covalent attachment method and non-reducing elution system yields pure co-IP products without contamination by the IP antibody
• Reusable antibody beads—covalent antibody immobilization allows prepared IP resin to be reused and provides potential savings of costly antibody
• Complete kit—all reagents for antibody immobilization and many IP experiments, including binding and wash buffers, elution buffer and convenient microcentrifuge spin columns to make resin manipulations easier and more efficient.
• Control beads included—underivatized agarose beads provided for use as negative control for nonspecific binding
• Versatile—co-IP method is compatible with any physiolgical (non-denaturing) protein sample buffer that is compatible with the specific antibody-antigen and protein interaction complex.

Contents

Sufficient For: Preparing and performing 50 co-IP reactions, each with 25 µL of resin

• AminoLink Plus Coupling Resin: 2 mL
• Coupling Buffer (20X): 25 mL
• Sodium Cyanoborohydride Solution (5M): 0.5 mL
• Quenching Buffer: 50 mL
• Wash Solution: 60 mL
• IP Lysis/Wash Buffer: 2 x 50 mL
• Modified Dulbecco's PBS (20X): 25 mL
• Conditioning Buffer (100X): 5 mL
• Elution Buffer: 50 mL
• Lane Marker Sample Buffer (5X): 5 mL
• Pierce Spin Columns, Screw Cap: 50 columns
• Microcentrifuge Collection Tubes, 2 mL: 100 tubes
• Microcentrifuge Collection Tubes, 1.5 mL: 50 tubes
• Pierce Control Agarose Resin: 2 mL