Thermo Scientific™ HisPur™ Cobalt Superflow Agarose, 50 mL

Thermo Scientific™ HisPur™ Cobalt Superflow Agarose, 50 mL

Thermo Scientific HisPur Cobalt Superflow Agarose is a tetradentate-chelating Superflow 6 support charged with divalent cobalt, designed for high-purity, large-scale purification of polyhistidine-tagged proteins.

Features

• Robust—highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200 cm/hour with minimal leaching of cobalt due to a tetradentate chelator
• High yields—binds 20 mg of 6xHis-GFP per mL of resin at a linear flow rate of 150 cm/hour, can bind greater than 30 mg per mL of resin depending on flow rate
• High purity—specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins
• Compatible—maintains function after exposure to a wide variety of chemicals and pH values
• Cost effective—resin is stable through multiple cycles of cleaning and reuse
• Stationary Phase: Metal Chelate, Divalent Cobalt
• Purification Target: His-tagged Proteins