Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose, 1 mL

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose, 1 mL

Thermo Scientific Pierce Anti-DYKDDDDK Magnetic Agarose provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins from in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to a magnetite-embedded agarose core particle.

Features

• Specific—unique base beads and highly specific antibody minimizes off-target binding (low non-specific binding)
• High purity—optimized bind-wash-elute protocol enables high purity
• High yield—special antibody conjugation method enables high yield
• Rapid—entire purification protocol typically takes less than 40 mins
• Economical—purification protocol allows multiple reuses
• Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)

Contents

• 1 mL settled beads. 4 mL supplied as a 25% suspension in PBS containing 0.02% sodium azide.